Analysis Note

ControlBrain tissue

Application

Immunohistochemistry at 1:100-1:200 for formaldehyde fixed tissue.

Optimal working dilutions must be determined by the end user.

Research CategoryNeuroscience

Anti-Serotonin Antibody, clone YC5/45 is an antibody against Serotonin for use in IH.

Research Sub CategoryNeurotransmitters & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Serotonin conjugated to BSA.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Lyophilized Supernatant, preservative free.

Unpurified

Specificity

Recognizes the formaldehyde conjugate of serotonin. Without paraformaldehyde treatment, it cross-reacts in liquid phase with dopamine and tryptamine in hemagglutination tests. Shows no reactivity with catecholamine-containing neurons in formaldehyde fixed preparations. MAB352 reacts with serotonin in neurons and recognizes serotonergic sites in fixed tissue sections. By cell agglutination, no cross reactivity with 5-hydroxyindolacetic acid, GABA, noradrenaline, 5-hydroxytryptophan, carnosine or melatonin.

Storage and Stability

Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100µL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.